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12 showing circulation times Tr vs. circulation time T=i~. - - 0 - - 0 - - 0 - - water; - - & - - & - - A - - carboxymethylcellulose,2%. The perfect distribution of the reaction mixture and the short mixing times in the compact lbop form are achieved by fast recirculation of the vessel content (Karrer 29~) has been well characterized hydrodynamically (for references, see Blenke 26)). First biotests reveal that productivity is, according to its low power input, rather modest. Sterility problems do not appear in the vessel itself, but in the external loop where appropriate types of pumps must be selected.

This is n o r m a l l y due to the presence o f inhibitors(s), recalcitrant substrates o r u n k n o w n limitations by nutrients. 38 A. J_.. c o. ~ , 42t ~8~ ~L ....... ,-, I 4~o ~go a~o t train) Fig. 18a. Regulatory pattern for malate dehydrogenase (MDH) activity in a pulse experiment with Saccharomyces cerevisiae. 10 h -1 and So = 2)~. Response of MDH-activity is quick indicating a mechanism acting on a allosteric level. Recovery from the pulse after exhaustion of the added glucose appears as a slowprocess.

Synopsis of media used in experimentation including mass culture of Escherichia coli: Included are 15 reports 99-1121 from the years 1951-1980. The list indicates the arbitrariness in the selection of elements, their forms, and concentrations of the carbon source. It also shows some attempts for supplementing some organic factors. 5 mg-3 g 21 Mg Na Ca Fe CI Trace elements Antifoam Physical and Chemical Parameters of Microbial Growth 45 A more difficult problem in the given approach for developing media composition, however, is finding data on the nature of molecules present only in extremely small quantities.

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